Imaging

Imaging: 2-Photon, Intrinsic and Low Photon Count, Bioluminescence, Wide Field

We use genetically encoded bioluminescent and fluorescent tags to visualize specific cells in the nervous system during behavioral and biological manipulations. Specifically, our lab works with fluorescent calcium and dopamine indicators. We also develop and implement novel bioluminescent molecules, such as calcium indicators, blood-brain barrier permeability indicators, and photo-switching molecules.

Imaging non-neuronal cells is one of the lab’s focuses. For example, in order to visualize brain vascular constriction and dilation we perfuse fluorescent dyes into the circulatory system. Using this method, we can relate vascular activity to blood brain barrier permeability, neuronal spiking, and behavior.